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Molecular Weight
Purity
Storage
Appearance
Solubility
01
Bronchogen is a synthetic tetrapeptide of alanine, aspartic acid, glutamic acid and leucine. It is one of the short-peptide bioregulators developed by the Khavinson group at the St Petersburg Institute of Bioregulation and Gerontology, studied in relation to bronchial epithelium.
The original papers on its DNA interactions write the sequence as Ala-Asp-Glu-Leu (ADEL), while some later papers from the same research network and many suppliers write Ala-Glu-Asp-Leu (AEDL). Both have the same formula and mass but are different molecules, so the order should be confirmed on the certificate of analysis. No CAS registry number has been assigned. It is supplied as a lyophilized reference material for in vitro and analytical research only.
02
Bronchogen is a small acidic tetrapeptide with a hydrophobic C-terminus.
Sequence
Ala-Asp-Glu-Leu (ADEL), as written in the primary DNA-interaction papers, with a free N-terminal amine and a free C-terminal acid. The alternative order Ala-Glu-Asp-Leu (AEDL) also appears in the literature and in supplier listings.
Charge
The aspartate and glutamate side chains plus the C-terminus give three negative charges at neutral pH, against one positive charge at the N-terminus, so the net charge is about −2.
Leu4
The C-terminal leucine adds some hydrophobic character, so Bronchogen is retained a little longer in reversed-phase HPLC than the other short peptides in this series.
No sensitive side chains
There is no aromatic residue, cysteine or methionine. The aspartate is the main site of chemical change.
03
Published research focuses on Bronchogen’s interaction with DNA, mostly from one research network.
DNA thermostability
Microcalorimetry studies reported that Bronchogen raised the melting temperature of calf-thymus and mouse-liver DNA by about 3.1 °C within a narrow range of peptide-to-base-pair ratios. The authors concluded that it binds both DNA strands, mainly through the bases, without clear preference for A–T or G–C pairs.
DNA methylation and nucleases
A 2011 study reported that Bronchogen changed how plant endonucleases cut methylated and unmethylated DNA in vitro, which the authors interpreted as sequence- or methylation-sensitive DNA binding.
Bronchial epithelium
Cell-culture work from the same group reported effects on proliferation and differentiation markers in bronchial epithelial cells.
Proposed mechanism
The group proposes that Bronchogen regulates gene expression by binding DNA directly. This is the group’s own framework and has not been widely confirmed independently.
These findings are cited as research context only; no effects are claimed for this material.
04
Peptide–DNA interaction assays
A reference compound in DNA melting (microcalorimetry), UV, circular dichroism and nuclease-protection experiments.
Bioregulator comparisons
Compared with Epitalon, Pinealon and other short peptides of the series in DNA-binding and cell studies.
Sequence-isomer analytics
Because ADEL and AEDL have identical mass, it is a useful test case for MS/MS methods that confirm residue order.
05
Storage
Store the lyophilized powder at −20 °C, sealed and desiccated.
Reconstitution
Highly soluble in water and aqueous buffers.
Chemical stability
The aspartate can form isoaspartate via an aspartimide, fastest in the mildly acidic range (around pH 4–6) and at higher temperatures, and Asp-containing bonds can be cleaved under strongly acidic conditions. Keep solutions near neutral pH, cold and fresh.
Solutions
Freeze single-use aliquots and avoid repeated freeze–thaw cycles.
06
Bronchogen’s identity depends on confirming the residue order.
HPLC
Reversed-phase C18 with a 0.1% TFA gradient starting at low organic content. It retains slightly longer than most short peptides of the series because of Leu4. Detect at about 205–214 nm.
Mass spectrometry
Positive-mode ESI gives [M+H]⁺ at m/z 447.2 (monoisotopic 446.20 Da). MS/MS shows the leucine immonium ion at m/z 86.1.
Confirming ADEL versus AEDL
Both orders have the same mass, but their fragments differ: the N-terminal b₂ ion is about m/z 187.1 for Ala-Asp (ADEL) and about m/z 201.1 for Ala-Glu (AEDL). MS/MS, or comparison with both reference standards by HPLC, confirms which isomer is present.
Impurity profile
Typical checks include isoAsp variants, truncated sequences, the other sequence isomer and counter-ion content.
07
Both appear in the literature. The original DNA-interaction papers write Ala-Asp-Glu-Leu (ADEL), while some later papers from the same research network and many suppliers write Ala-Glu-Asp-Leu (AEDL). They are different molecules with the same formula, so confirm the order on the certificate of analysis.
No CAS registry number has been assigned. It is identified by its sequence, formula (C₁₈H₃₀N₄O₉) and mass.
Its developers propose that it binds DNA directly and so influences gene expression. Supporting evidence includes increased DNA melting temperature and changed nuclease cleavage in vitro, but this framework comes mainly from one research group and has not been widely confirmed independently.
By MS/MS: the b₂ fragment is about m/z 187.1 for ADEL (Ala-Asp) and about m/z 201.1 for AEDL (Ala-Glu). HPLC comparison against both reference standards also works.
Around 205–214 nm. It has no aromatic residues, so 280 nm gives no useful signal.
No. It is supplied strictly as a reference compound for laboratory and analytical research and is not for human or animal use.
Bronchogen Research Reference. (2026). Pure Peptides Labs Research Catalogue. Retrieved from https://purepeptideslabs.net/research-catalogue/cellular-bioregulator-reference-compounds/bronchogen/
Materials listed on this platform are supported by independent testing and published documentation for laboratory research contexts.
